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A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins.

A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Research Abstract Details 

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  • A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Abstract Text:

    yingqi xuYingqi Xu,zhi linZhi Lin,chien hoChien Ho,daiwen yangDaiwen Yang,

    A general strategy is proposed to assign aliphatic side-chain resonances of large 13C,15N-labeled proteins without deuteration, using 4D 13C,15N-edited NOESY and MQ-(H)CCH-TOCSY experiments on the basis of prior assignments of backbone and 13Cbeta resonances. The strategy has been tested on a 214 residue protein (DdCAD-1) and applied to a chain-selectively 13C,15N-labeled hemoglobin (65 kDa). About 96 and 80% aliphatic side-chain spins in DdCAD-1 and hemoglobin have been assigned, respectively. The strategy proposed here will be very useful for the structure determination and dynamics characterization of large proteins by NMR.

    A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Publishing Authors By Initials

    y xuY Xu,z linZ Lin,c hoC Ho,d yangD Yang,

    For similar proteins research abstracts see: proteins research

    PUBMED ID PMID:

    MEDLINE DATE:

    A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of the American Chemical Society

    VOLUME: 127

    Page Numbers: 11920-1

    Journal Abbreviation: J. Am. Chem. Soc.

    ISSN: 0002-7863

    DAY: 31

    MONTH: Aug

    YEAR: 2005

    A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 7503056

    A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Keywords Mesh Terms:

    KEYWORDS: Proteins

    MESH TERMS: chemistry

    Chemical & Substance for Abstract: A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins. Information

    Substance Name: Proteins

    Registry Number: 0

    Grant and Affiliation Information for A general strategy for the assignment of aliphatic side-chain resonances of uniformly 13C,15N-labeled large proteins.

    AFFILIATION: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543.

    Country: United States

    United States Research PublicationUnited States Research Publication

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    ACRONYM:

    MEDLINETA: J Am Chem Soc

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