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A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor.

A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Research Abstract Details 

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  • A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Abstract Text:

    margot g paulickMargot G Paulick,martin b forstnerMartin B Forstner,jay t grovesJay T Groves,carolyn r bertozziCarolyn R Bertozzi,margot g paulickMargot G Paulick,martin b forstnerMartin B Forstner,jay t grovesJay T Groves,carolyn r bertozziCarolyn R Bertozzi,

    The glycosylphosphatidylinositol (GPI) anchor is a C-terminal posttranslational modification found on many eukaryotic proteins that reside in the outer leaflet of the cell membrane. The complex and diverse structures of GPI anchors suggest a rich spectrum of biological functions, but few have been confirmed experimentally because of the lack of appropriate techniques that allow for structural perturbation in a cellular context. We previously synthesized a series of GPI anchor analogs with systematic deletions within the glycan core and coupled them to the GFP by a combination of expressed protein ligation and native chemical ligation [Paulick MG, Wise AR, Forstner MB, Groves JT, Bertozzi CR (2007) J Am Chem Soc 129:11543-11550]. Here we investigate the behavior of these GPI-protein analogs in living cells. These modified proteins integrated into the plasma membranes of a variety of mammalian cells and were internalized and directed to recycling endosomes similarly to GFP bearing a native GPI anchor. The GPI-protein analogs also diffused freely in cellular membranes. However, changes in the glycan structure significantly affected membrane mobility, with the loss of monosaccharide units correlating to decreased diffusion. Thus, this cellular system provides a platform for dissecting the contributions of various GPI anchor components to their biological function.

    A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Publishing Authors By Initials

    mg paulickMG Paulick,mb forstnerMB Forstner,jt grovesJT Groves,cr bertozziCR Bertozzi,mg paulickMG Paulick,mb forstnerMB Forstner,jt grovesJT Groves,cr bertozziCR Bertozzi,

    For similar abstracts research abstracts see: abstracts research

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    A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Journal Published:

    PUBLICATION TYPE: Research Support, U.S. Gov't,

    Journal: Proceedings of the National Academy of Sciences of

    VOLUME: 104

    Page Numbers: 20332-7

    Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

    ISSN: 1091-6490

    DAY: 12

    MONTH: 12

    YEAR: 2007

    A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 7505876

    A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Keywords Mesh Terms:

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    Grant and Affiliation Information for A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor.

    AFFILIATION: Departments of Chemistry and Molecular and Cell Biology, and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.

    Country: United States

    United States Research PublicationUnited States Research Publication

    AGENCY: United States NIGMS

    GRANT: GM59907

    ACRONYM: GM

    MEDLINETA: Proc Natl Acad Sci U S A

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