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Western Blot Analysis of Endogenous Gene Expression Protocol - Details

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Protocols » Listing Details
ID:3422
Title: Western Blot Analysis of Endogenous Gene Expression Protocol - http://www.flemingtonlab.com/Protocols/WestBlotAnalyofEndogProts.pdf
Description:Procedure describes how it denatures most of the modification and degradation proteins immediately giving the most accurate read out of the true levels of protein at the time of harvest.  However, in cases where detection is a problem, a limited purification (e.g. isolation of nuclear extract for the detection of transcription factors) might be required to allow analysis.
Category:Western Blot Protocols
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Date Added:May 10, 2008 02:01:14 AM
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