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Immunoblotting: Preparing Immunoprecipitated Proteins Protocol - Details

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Protocols » Listing Details
ID:3421
Title: Immunoblotting: Preparing Immunoprecipitated Proteins Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4300
Description:Protein immunoprecipitation can be a useful preparative step for immunoblotting.  For very rare proteins, the protein of interest can be purified and concentrated by standard immunoprecipitation techniques before immunoblotting.  In addition, protein-protein interactions can be tested with an immunoprecipitating antibody that is specific for one protein of a complex and an immunoblotting antibody that is specific for a second member of the complex.
Category:Western Blot Protocols
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Date Added:May 10, 2008 01:57:18 AM
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