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Long and Accurate PCR Protocol - Details

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Protocols » Listing Details
Title: Long and Accurate PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4094
Description:Protocol illustrates the rules of successful long PCR: No more than 1 ng of template DNA is used per microliter of PCR in a 100-µl reaction; approximately 0.1 µl of KlentaqLA (not plain Taq) is used per kilobase of target (for targets >10 kb, 1-1.3 µl of enzyme should be used); the Mg++ concentration is considered as the excess over the level of dNTPs.
Category:PCR Protocols
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Date Added:April 12, 2008 07:14:01 AM
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