Genetic Engineering with PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3836
Description:
Method describes how to modify the termini of PCR products by introducing restriction sites and other features. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. Bake all glassware for 6 hours at 150°C and autoclave all plasticware.
