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Differential Display-PCR Protocol - Details

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Protocols » Listing Details
Title: Differential Display-PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3844
Description:Method uses PCR to amplify and display many cDNAs derived from the mRNAs of a given cell or tissue type.  The method relies on two different types of synthetic oligonucleotides: anchored antisense primers and arbitrary sense primers.  A typical anchored primer is complementary to approx. 13 nucleotides of the poly(A) tail of mRNA and the adjacent two nucleotides of the transcribed sequence.
Category:PCR Protocols
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Date Added:April 12, 2008 07:09:33 AM
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