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siRNA Double Transfection of RAW 264.7 Cells with Lipofectamine Protocol II - Details

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Protocols » Listing Details
ID:3186
Title: siRNA Double Transfection of RAW 264.7 Cells with Lipofectamine Protocol II - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000262.pdf?pid=PP00000262
Description:Protocol Involves the transfection of siRNA into RAW 264. 7 cells using Lipofectamine 2000.  Cells are transfected with siRNA twice (on subsequent days).  Target gene knockdown is assessed from total RNA isolated 48 hr post-transfection or from protein isolated 72 hr post-transfection.
Category:RNAi Technology Protocols
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Date Added:March 19, 2008 07:50:43 AM
Number Hits:216
 

 

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