siRNA Double Transfection of RAW 264.7 Cells with Lipofectamine Protocol II - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000262.pdf?pid=PP00000262
Description:
Protocol Involves the transfection of siRNA into RAW 264. 7 cells using Lipofectamine 2000. Cells are transfected with siRNA twice (on subsequent days). Target gene knockdown is assessed from total RNA isolated 48 hr post-transfection or from protein isolated 72 hr post-transfection.
