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Annealing, Cloning, and Sequencing of shRNA Linkers into pEN_H1 Plasmids Protocol - Details

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Protocols » Listing Details
ID:3179
Title: Annealing, Cloning, and Sequencing of shRNA Linkers into pEN_H1 Plasmids Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000230.pdf?pid=PP00000230
Description:Protocol describes the preparation of an shRNA-expressing Gateway entry vector.  This process is preceded by the design and synthesis of target gene-specific sense and antisense oligonucleotides which, when annealed, will constitute an shRNA cassette.
Category:RNAi Technology Protocols
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Date Added:March 19, 2008 07:40:23 AM
Number Hits:211
 

 

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