The siRNA User Guide - http://www.rockefeller.edu/labheads/tuschl/sirna.html
Description:
The siRNA user guide. Includes: Selection of siRNA duplexes from the target mRNA sequence; Preparation of siRNA duplexes; Useful siRNA reagent combinations; Transfection of siRNA duplexes; Sequences of siRNA duplexes used in our studies.