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Ligating Plasmid and Target DNAs in Low-melting-temperature Agarose Protocol - Details

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Protocols » Listing Details
ID:3001
Title: Ligating Plasmid and Target DNAs in Low-melting-temperature Agarose Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3929
Description:Protocol for ligating plasmid and target DNAs in low-melting-temperature agarose. Ligation in low-melting-temperature agarose is much less efficient than ligation with purified DNA in free solution and requires a large amount of DNA ligase. The method is used chiefly for rapid subcloning of segments of DNA in dephosphorylated vectors and assembling recombinant constructs.
Category:Cloning Protocols
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Date Added:February 21, 2008 09:19:25 PM
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