Attaching Adaptors to Protruding Termini Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3920
Description:
Adaptors are short double-stranded synthetic oligonucleotides that carry an internal restriction endonuclease recognition site and single-stranded tails at one or both ends. Adaptors are used to exchange restriction sites at the termini of linear DNA molecules. They may be purchased in phosphorylated and unphosphorylated forms.