Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

PCR Troubleshooting

Polymerase Chain Reaction Problems and Solutions

This is a troubleshooting page for common PCR problems and their solutions.

PCR Problems & Troubleshooting Guides

Long Non-Specific PCR Products

PCR Primer Dimers

PCR No Bands

PCR Non Specific Product

 

Factors for a Successful PCR

Can't Find Your Problem? Make sure you see the PCR Forum for PCR troubleshooting and solutions to common problems. You can also post questions to other researchers by simply registering and then clicking on Post New Thread or the question mark below!

PCR Forum Topics

loading dye Has anybody ever come across a loading dye that ran at a different molecular size on an agarose gel than expected? Why is this happening?
Cresol Red/Sucrose Hi. Does anyone have experience using cresol red/sucrose as loading dye added to the reaction BEFORE PCR? Does cresol red/sucrose have to have a...
Amplification won't work! I'm trying to amplify a ~200bp segment from a ~4kb plasmid. A postdoc in my lab previously amplified the segment and gave me a stock of gel-extracted...
Any ideas why my PCR amplification won't work? I'm trying to amplify a ~200bp segment from a ~4kb plasmid. A postdoc in my lab previously amplified the segment and gave me a stock of gel-extracted...
Heated lid on RFLP: is it ok? I'm doing three different RFLPs with three different restriction enzymes using a single amplicon, each RFLP in different tubes of course as each...
You must REGISTER NOW to post a question in the PCR Forum by clicking here.

View More PCR discussions and troubleshooting in the: PCR Forum

 

PCR NEWSLETTER GROUP!!

Master the PCR Technique with our PCR Newsletter covering the latest PCR methods, Protocols, Discussions and Troubleshooting, and PCR products to help you with your PCR!

FirstName:
Email:

Note: We will only send you PCR related information not spam.We hate spam as much as you do and You can unsubscribe at any time.

Science News

For science news click here:Science News