Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Autoclave Operation

Procedure for Operating the Autoclave

Material to be autoclaved is placed in a autoclavable tray. The autoclaved items must have indicator tape. It is necessary to separate liquids from solids and then autoclave separately. Importantly all lids on all bottles are adjusted to be loose. Avoid crowding large number of items on the tray, this then allows for all items to be autoclaved to reach the appropriate temperature, one must allow sufficient air/steam circulation.


1. Chamber pressure should be at 0 before opening the door- Make sure of this.

2. Items that require autoclaving should be placed on the tray and in the autoclave machine afterwards closing the door.

3. Some autoclave machines require that you also lock the door after it's closed.

4. Set time: usually set for 20 minutes.

5. Temperature should be set at 121EC already, but double-check and change if necessary.

6. Set cycle: If liquid, set "liquid cycle" or "slow exhaust". If dry, set "dry cycle" or "fast exhaust" + dry time.

7. Start the cycle. On some autoclaves, the cycle starts automatically at step 5. On others, turn to "sterilize".


1. The chamber pressure is at 0

2. . the temp is <100EC

3. Open door. (On 3rd floor autoclave, don't push end cycle)

4. Remove contents using gloves and immediately tighten all caps.

Autoclaving Forum Topics

Questions & Troubleshooting Forum Discussions

You must REGISTER NOW to post a question in the Microbiology Forum. Login now if you have already registered.

Molecular Biology Products & Services

Research and Laboratory Products and Vendors Directory - organized by topic. Companies, list Your products and company in the product directory for FREE by Registering Your Company Here!

  • Antibody Products
  • Lab Equipment
  • Peptide Products
  • Much More Coming Soon!
  • Microbiology Newsletter

    Yes! I Want to Learn the Latest in microbiology, fungal, bacteriology and virology-related Research and receive Email Alerts on new microbiology culture products and services!
    Don't Worry Your Email is Safe with Us. We hate Spam as Much as You Do.  You can unsubscribe at any time by clicking on unsubscribe on the bottom of Every Email!
    First Name:

    Science News

    For science news click here:Science News