Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Aeration & Soil Microorganisms Bacteria

Information on the Aeration of Soil and the Growth of Soil Bacterial Cultures

Table of Contents:

Bacterial Culture Troubleshooting and Forum Topics

Related Microbiology Articles

Join the Microbiology Newsletter!

Exercise Experiment - An Illustration of the Effect of Soil Aeration and the Activity of Microorganisms Present

The Effect of Aeration of Soils and Bacteria

Apparatus Needed

Coarse, medium, and fine sand; magnesium oxide; dilute HC1; N/10 NH 4 OH; N/10 HC1; indicator; sterile 1 c.c. pipette; ten sterile 250 c.c. Erlenmeyer flasks; condenser; sterile 1% peptone solution.

Culture

B. mycoides, broth culture.

Method or Protocol

1. Prepare the sand for use by first heating it with dilute HC1; then wash it several times, first with tap water then with distilled water and dry at 110 C.

2. Place 50 gms. Of coarse sand in each of three flasks; do the same with the medium and the fine sand.

3. Sterilize the flasks in the autoclav at 120 C. for thirty minutes.

4. Place 50 c.c. of the 1% peptone solution in the remain ing flask.

5. Add sufficient peptone solution to the other flasks to make 10%, 20% and 30% moisture content according to the following table :

Soil.

Moisture.

After twenty days.

Coarse sand \

(

10% 20% 30%

Medium sand -\

10%

20%

30%

Fine sand !

10%

20%

1% peptone solution

30% 99%

6. Inoculate each flask with 1 c.c. from a broth culture of B. mycoides and shake to distribute the organisms evenly.

7. Make ammonia determinations after twenty days.

Note. Ammonia determinations from the above are made by the following procedure: Remove the cotton plugs, add 200 c.c. of water, 10 gms. MgO and a small piece of paraffin. Distill off the ammonia present, collecting it in N/10 HC1 and titrating against N/10 NH 4 OH, using methyl orange as indicator.

8. Record your results in the form given above.

9. How does aeration affect bacterial activity? Size of sand grains?

What interrelation have grain-size and moisture con tent of soil?

How do microorganisms obtain their food in soil that is not wet?

What influence does humus have on aeration? On bacterial activity?

10. Draw any conclusions warranted by your results and point out any practical applications.

References for Soil Aeration & Microorganisms

RAHN, O.: Tech. Bui. No. 6, Mich. Agr'l Expt. Sta.

Microbiology Forum Topics

fusobacterium culturing hello everybody i need a help on how to eliminate the growth of colon bacteria on sheep blood agar ? i'm looking for fusobacterium that i have...
Plate streaking order to avoid media crossover. I feel almost embarrassed to ask this mainly because a few years ago I could of answered this question in my sleep and is very basic, but seems...
drank fountain water in Rome.. parasitic infection? Ok this is a long shot, but this forum seemed like a good place to ask.. To cut a long story very short I went on holiday to Rome with a complete...
Correct storage of cells protocol help. Hi everyone, I am having the correct storage of my cells. I am growing M. tuberculosis to McFarland 1 then I store them as aliquots in eppies at...
Help with some basic micro protocols Basically my experiment looks like this: (1) Culture on LJ's (2 weeks @ 37 degrees) -> (2) transfer colony (aseptically) to 10 - 20ml 7H9 media with...
You must REGISTER NOW to post a question in the Microbiology Forum. Login now if you have already registered.

Molecular Biology Products & Services

Research and Laboratory Products and Vendors Directory - organized by topic. Companies, list Your products and company in the product directory for FREE by Registering Your Company Here!

  • Antibody Products
  • Lab Equipment
  • Peptide Products
  • Much More Coming Soon!
  • Microbiology Newsletter

    Yes! I Want to Learn the Latest in microbiology, fungal, bacteriology and virology-related Research and receive Email Alerts on new microbiology culture products and services!
    Don't Worry Your Email is Safe with Us. We hate Spam as Much as You Do.  You can unsubscribe at any time by clicking on unsubscribe on the bottom of Every Email!
    First Name:
    Email:

     

     

    Science News

    For science news click here:Science News