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#1 01-23-2009, 12:33 AM

I*am looking to identify specific bacterial metabolites that cause malodor and then measure those byproducts by GC or other analytical method.* But I am finding it difficult to find those specific by-products.* Most interested in by-products of Staph and E. coli.* We have looked at ammonia and Proteus, measuring with Drager columns, but I want something more sophisticated.* Any ideas?

#2 01-23-2009, 08:51 PM

On Thu, 22 Jan 2009 17:33:00 -0800 (PST), Kate Hawley
<[Only registered users see links. ]> wrote:

Take a culture that you find malodorous. Run the headspace gas thru
GC-MS.

bob

#3 01-25-2009, 11:41 AM

On Jan 24, 5:51*am, Bob <[Only registered users see links. ].com> wrote:

Remove the contaminating dominant products first through depletion via
negative column-exchange chromatography. Then do GC-MS as Bob
suggested.

sz

Jorge1907 · #4 01-26-2009, 01:00 PM

Do some homework 1st. You're nt the 1st to have considered this.. For the E. coli - think skatole, indole, H2S. For staph consider volatile fatty acids.

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