Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Immunology and Host-Pathogen Interactions
Register Search Today's Posts Mark Forums Read

Splenic adherent cells wont adhere!

Splenic adherent cells wont adhere! - Immunology and Host-Pathogen Interactions

Splenic adherent cells wont adhere! -

LinkBack Thread Tools Display Modes
Old 10-30-2009, 03:22 PM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
Join Date: Oct 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Splenic adherent cells wont adhere!

Hi, all
I am newbie to immunology and trying to culture mouse spleen adherent cells to study cytokine production upon LPS stimulation.

I dissect spleen, mechanically dissociate using the end of plunger, and plate onto petridish/culture dish in RPMI1640/Heat Inactivated 10% New born Calf Serum/Pen/Strep for one hour.

After one hour incubation, I can see lots of cells adhere. So I wash 3 times to remove non-adherent cells and incubate overnight. Then for some reason almost all cells come off the plate and not much left on the plate!

Please Immune expert here could you provide any solution/mistake I am making?
Thank you veery much in advance!

Reply With Quote

adhere , adherent , cells , splenic

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 12:52 PM
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-27-2011 12:40 AM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 03:18 PM
How to isolate adult from circulation? peterish Stem Cell Forum 0 05-19-2008 11:02 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM

All times are GMT. The time now is 04:42 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2013, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.20388 seconds with 16 queries