Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum - Cell Biology and Cell Culture http://www.molecularstation.com/forum/ Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology. en Thu, 15 Aug 2013 18:02:19 GMT vBulletin 60 http://www.molecularstation.com/forum/images/misc/rss.jpg Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum - Cell Biology and Cell Culture http://www.molecularstation.com/forum/ B cell culture. Question!! http://www.molecularstation.com/forum/cell-biology-cell-culture/87985-b-cell-culture-question.html Sun, 07 Jul 2013 10:11:37 GMT Hi, I am new in the field of cell culture. I am performing an experiment to detect IgE production in the supernatants of isolated and stimulated B...
Hi,

I am new in the field of cell culture. I am performing an experiment to detect IgE production in the supernatants of isolated and stimulated B lymphocytes. According to my protocol I should take the supernatants from my cell culture (I plate 100.000 B cells in 100 microlitres in 96 well plate) at day 14 in order to analyze the IgE. But at that day the medium is completely yellow.

Does anybody have any experience on measuring IgE in B cell culture supernatants? Is day 14 already too late? The medium is yellow so, I am afraid most of the cells are already dead at that time.

I would appreciate you share your experiences with me.

Thank you,

YM
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Cell Biology and Cell Culture Arwen http://www.molecularstation.com/forum/cell-biology-cell-culture/87985-b-cell-culture-question.html
<![CDATA[I've no experience .. please help me]]> http://www.molecularstation.com/forum/cell-biology-cell-culture/87906-ive-no-experience-please-help-me.html Sun, 02 Jun 2013 23:07:25 GMT > I want ur help I am Student at Master's Degree in Cell biology, Histology and Genetics My master's research point on 2 species of...]]>
Hi guys >>
I want ur help
I am Student at Master's Degree in Cell biology, Histology and Genetics

My master's research point on 2 species of crab

( Portunus pelagicus , and Charybdis helleri )

under title " Ecological and Biological Studies on Some Alien Crab Species in Egyptian Mediterranean Waters"

we collect specimens from red and Mediterranean seas seasonally and study them in comparative way

My problem is i want to link histology with climate change may be climate change or the environment of the red sea differ from that of medit. and them affect the crab

>> But I am fear from the results .. may be i will not find differences.

So every one has an idea .. how i can put the histology in the research .. even if from another point of view >>>>>

Thanks in advance for all of u :D
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Cell Biology and Cell Culture Marwa Ismaiel http://www.molecularstation.com/forum/cell-biology-cell-culture/87906-ive-no-experience-please-help-me.html
Histone H1 isolation - avoiding acidic conditions http://www.molecularstation.com/forum/cell-biology-cell-culture/87905-histone-h1-isolation-avoiding-acidic-conditions.html Sun, 02 Jun 2013 19:12:51 GMT Dear all, First of all I have to say that I am not really experienced with cells and proteins isolation. My question is that I wanna isolated...
Dear all,

First of all I have to say that I am not really experienced with cells and proteins isolation.

My question is that I wanna isolated Histone H1 from a type of cells. There are a lot of reports, but all of them used acidic conditions (perchlorid acid, sulfuric acid, trichloroacetic acid...) and thats something that I would like to avoid. In some of the cases I understand that such conditions are used to precipitate the proteins and in others I guess that are to break/lyse the cell membrane nucleus.

Are u aware of any method to isolate proteins from the nucleus avoiding acidic conditions?

And second question, why such acids are being used to isolated such proteins (in case that my previous afirmations are wrong)?

Thanks very much!
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Cell Biology and Cell Culture Valine http://www.molecularstation.com/forum/cell-biology-cell-culture/87905-histone-h1-isolation-avoiding-acidic-conditions.html