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Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule.

Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule. Research Abstract Details 

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  • Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule. Abstract Text:

    m kawanishiM Kawanishi,t yoshidaT Yoshida,m kijimaM Kijima,k yagyuK Yagyu,t nakaiT Nakai,s okadaS Okada,a endoA Endo,m murakamiM Murakami,s suzukiS Suzuki,h moritaH Morita,m kawanishiM Kawanishi,t yoshidaT Yoshida,m kijimaM Kijima,k yagyuK Yagyu,t nakaiT Nakai,s okadaS Okada,a endoA Endo,m murakamiM Murakami,s suzukiS Suzuki,h moritaH Morita,

    AIMS: To identify Lactococcus garvieae isolates from radish and broccoli sprouts and compare them with virulent and less virulent mutant strains obtained from yellowtails with regard to KG phenotype, presence of a capsule and virulence towards yellowtails and mice. METHODS AND RESULTS: Comparative 16S rRNA gene sequence analysis of six isolates obtained from radish and broccoli sprouts indicated that they were L. garvieae (similarity >99%). They were compared with KG9502, Lg2 and ATCC49156 strains obtained from yellowtails. A less virulent mutant strain Lg2-S was obtained by Lg2 subculture. Biochemical characterization of the six strains resembled that of KG9502, Lg2, ATCC49156 and Lg2-S, except for saccharose and tagatose acidification and the presence of hippuricase. These six strains were nonpathogenic towards yellowtails and mice, nonsusceptible to bacteriophages and demonstrated heterogeneity on pulsed-field gel electrophoresis analysis. Using transmission electron microscopy, a capsule was observed in KG9502 and Lg2 but not in ATCC49156 and Lg2-S. CONCLUSIONS: We isolated L. garvieae strains that lacked pathogenicity towards yellowtails and mice from radish and broccoli sprouts; these were noncapsulated and exhibited KG(+) phenotype. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first documentation of L. garvieae isolated from terrestrial plants. These isolates exhibited genetic diversity; however, they were noncapsulated and nonpathogenic towards yellowtails and mice.

    Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule. Publishing Authors By Initials

    m kawanishiM Kawanishi,t yoshidaT Yoshida,m kijimaM Kijima,k yagyuK Yagyu,t nakaiT Nakai,s okadaS Okada,a endoA Endo,m murakamiM Murakami,s suzukiS Suzuki,h moritaH Morita,m kawanishiM Kawanishi,t yoshidaT Yoshida,m kijimaM Kijima,k yagyuK Yagyu,t nakaiT Nakai,s okadaS Okada,a endoA Endo,m murakamiM Murakami,s suzukiS Suzuki,h moritaH Morita,

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    Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Letters in applied microbiology

    VOLUME: 44

    Page Numbers: 481-7

    Journal Abbreviation:

    ISSN: 0266-8254

    DAY: 24

    MONTH: May

    YEAR: 2007

    Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule. Information

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    LANGUAGE: eng

    NlmUniqueID: 8510094

    Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule. Keywords Mesh Terms:

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    Grant and Affiliation Information for Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule.

    AFFILIATION: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo, Japan. michiko_kawanishi@nm.maff.go.jp

    Country: England

    England Research Publication

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    MEDLINETA: Lett Appl Microbiol

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