Epigenetics and Methylation Protocols

A practical guide to DNA methylation Promega PDF The pattern and extent of DNA methylation can significantly affect the success of restriction digestions or bacterial transformations. Prokaryotic DNA may be methylated by host restriction/modification systems, while eukaryotic DNA often is methylated i

Bisulfite Treatment of DNA Anderson Lab Bisulfite Treatment of DNA Anderson Lab. Adapted from Frommer et.al.

Bisulfite-PCR for Restriction Analysis and/or Sequencing Bisulfite-PCR for Restriction Analysis and/or Sequencing. Protocol written by Jean-Pierre Issa. M.D Anderson Cancer Centre Texas.

DNA-Methyltransferase Assay - Anderson Centre M.D. Anderson Cancer Centre Texas

Methylated CpG Island Amplification Methylated CpG Island Amplification. Protocol written by Minoru Toyota. M.D Anderson Cancer Centre Texas.

Methylation Specific PCR Protocol Methylation Specific PCR Protocol. M.D Anderson Cancer Centre Texas. Protocol written by James Herman*

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

[Read more]