Epigenetics and Methylation Protocols Protocol Links
A practical guide to DNA methylation Promega PDF The pattern and extent of DNA methylation can significantly affect the success of restriction digestions
or bacterial transformations. Prokaryotic DNA may be methylated by host restriction/modification
systems, while eukaryotic DNA often is methylated i
Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.