EMSA DNA-Protein Protocols

EMSA Protocols for DNA-binding protein assessment using Electrophoretic Mobility Shift Assay.

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EMSA DNA-Protein Protocols Protocol Links

A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE) A non-radioactive electrophoretic mobility shift assay based on the digoxigenin detection system had been developed and applied to the analysis of the interaction between the heat shock transcription factor and the heat shock element of N. crassa. (Bioch

DNA-Binding Protein Purification with Dynabeads. Magnetic DNA affinity purification of yeast transcription factor tau--a new purification principle for the ultrarapid isolation of near homogeneous factor. Gabrielsen et al. 1989

DNaseI Footprinting Assay DNaseI Footprinting Assay. Works well high affinity dna binding proteins. Breeden Lab.

Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB. CellDeath.de

EMSA protocol (Word Doc) Short EMSA protcol starting with gel running and supershift assay. Hammer Lab.

EMSA Protocol using 32-p. Primer design, Primer annealing, Primer labelling, Probe purification, In vitro hybridization DNA-protein Binding reaction. Jonathan Flint.

EMSA using ds Oligonucleotides EMSA probe creation using ds Oligonucleotides by annealing two complementary oligos, and Klenow for probe creation.

Gel Mobility Shift Assay in PDF format This protocol works well for HSF DNA-binding activity experiments.

General EMSA Protocol There are multiple variations to this protocol, but they find that this one works well in all cases tested. Mirmira Lab.

Introduction to the EMSA (Gel Shift) Technique A non-radioactive electrophoretic mobility which works quite well using biotin and streptavidin detection. Non-specific and specific competititor, oligo labeling, Binding Reaction. Pierce

Nuclear Extract Preparation for EMSA (PDF) Nuclear Extract Preparation for EMSA after Wadman et al., EMBO J. 1997. Buffer recipies included.

Protocol for Non-Radioactive EMSAs Using DIG Very Nice and Detailed Protocol for Non-Radioactive EMSAs Using DIGoxin. Hammer Lab.

Radiolabeling of Probes for Electrophoretic Mobility Shift Assay Creation of Probe for EMSA using Oligonucleotide annealing, p-32, and T4 Polynucleotide Kinase.