DNA-Binding Protein Purification with Dynabeads. Magnetic DNA affinity purification of yeast transcription factor tau--a new purification principle for the ultrarapid isolation of near homogeneous factor. Gabrielsen et al. 1989
DNaseI Footprinting Assay DNaseI Footprinting Assay. Works well high affinity dna binding proteins. Breeden Lab.
EMSA protocol (Word Doc) Short EMSA protcol starting with gel running and supershift assay. Hammer Lab.
EMSA Protocol using 32-p. Primer design, Primer annealing, Primer labelling, Probe purification, In vitro hybridization DNA-protein Binding reaction. Jonathan Flint.
EMSA using ds Oligonucleotides EMSA probe creation using ds Oligonucleotides by annealing two complementary oligos, and Klenow for probe creation.
General EMSA Protocol There are multiple variations to this protocol, but they find that this one works well in all cases tested. Mirmira Lab.
Introduction to the EMSA (Gel Shift) Technique A non-radioactive electrophoretic mobility which works quite well using biotin and streptavidin detection. Non-specific and specific competititor, oligo labeling, Binding Reaction. Pierce
