Fam Caspase 8 and 9 Binding Assay for Embryos Protocol The FAM caspase binding assay kits from ATCC Corporation can be used to determine amounts of active caspases in cells. The FAM-labeled caspase inhibitor can freely diffuse into the cell. Active caspase irreversibly binds the inhibitor. Upon washing the cells, the amount of fluorescence is proportional to the amount of active caspase in the cell. FAM-LETD-fmk (catalog no. 30-1306) is used to detect caspase 8 and FAM-LEHD-fmk (catalog no. 30-1308) is used for caspase 9.
Mini-Chamber for Regulating Gaseous Environment During Culture A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device.
Procedures for In Vitro Production of Bovine Embryos Procedures for in vitro production of bovine embryos. Includes: Collection of Ovaries; Oocyte Collection; Preparation of IVF Medium; In Vitro Fertilization; Culture of Embryos.
Protocol to Count Cell Number of Preimplantation Embryos Protocol to Count Cell Number of Preimplantation Embryos
using Nuclear Staining with Hoechst 33342 or DAPI. Includes: Preparation of Embryos; Preparation of Hoechst 33342 dye; Preparation of DAPI; Staining the Embryo; Mounting Embryos to Slides; What to Do When There are Too Many Cells to Count.
Visualizing Early Embryo Implantation Sites by Dye Injection Protocol This protocol describes a method for visualizing early embryo implantation sites using Chicago Sky Blue 6B dye. Once implantation and interimplantation sites are identified and separated, they can be used for cellular, biochemical, and molecular biology analyses.
