Electroporation Sonoporation Protocols

Bacterial Transformation Electroporation Protocol Protocol for bacterial transformation electroporation. Includes: Preparation of Electrocompetent cells.

Electroporation and RNAi in the Rodent Retina In Vivo and In Vitro Protocol Protocol for electroporation and RNAi in the rodent retina in vivo and in vitro.

Electroporation for Sindbis Virus Production Protocol Protocol for electroporation for Sindbis virus production.

Electroporation of Burkitt's Lymphoma Cell Lines Protocol Protocol for electroporation of Burkitt's Lymphoma cell lines.

Electroporation of ES Cells Protocol Protocol for the electroporation of Es cells.

Electroporation of Hela Cells Protocol Protocol for the electroporation of Hela cells.

Electroporation of U2OS Cells Protocol Protocol for the electroporation of U2OS cells.

Electroporation Protocol Protocol for electroporation.

Optimizing Electrotransfection of Mammalian Cells In Vitro Protocol Protocol describes transfection of plasmid DNA into mammalian cell lines using electroporation, a process whereby external application of electric pulses induce cell membrane permeability. A number of factors can affect electrotransfection efficiency. In general, cells in suspension and small volume cells are difficult to transfect, whereas adherent cells and large volume cells are relatively easy.

Preparation of Electrocompetent E. coli DH5 Protocol Protocol for the preparation of electrocompetent E. coli (i.e. DH5 ).

Protocol Electroporation Protocol describes a method for introducing gene constructs into mouse embryos by electroporation. Gene constructs can be quickly tested for tissue-specific transcriptional activity or can be used to overexpress gene products.

Protocol Transformation of E. coli by Electroporation Protocol for the transformation of e. coli by electroporation.

Transformation of E. coli by Electroporation Protocol Protocol for the transformation of e. coli by electroporation.

Transformation of E. Coli by Electroporation Protocol.

Electrocompetent bacteria are prepared by growing cultures to mid-log phase, washing the bacteria extensively at low temperature, and then resuspending them in a solution of low ionic strength containing glycerol. DNA is introduced during exposure of the bacteria to a short high-voltage electrical discharge.

Transformation of Tetrahymena thermophila by Electroporation Protocol Protocol describes a method for transformation of the Tetrahymena using electroporation. The vector is electroporated into cells after mating, where it is incorporated into the DNA of developing macronuclei. Because T. thermophila can be propagated indefinitely without conjugation, transformation of the macronucleus provides a way to obtain stable somatic transformants.