E coli Transformation Protocols

Electro Competent Cells Protocol Protocol for electro competent cells.

Frozen Competent E. coli Preparation and Transformation Protocol Protocol for frozen competent E. coli preparation and transformation.

Preparation and Transformation of Competent E. coli Protocol Procedure generates competent cultures of E. coli that can be transformed at high frequencies (5 x 108 transformed colonies/µg of superhelical plasmid DNA). IMPORTANT All steps in this protocol should be carried out aseptically.

Preparation and Transformation of Competent E. coli Using Calcium Chloride Protocol Protocol is is used to prepare batches of competent bacteria that yield 5 x 106 to 2 x 107 transformed colonies/µg of supercoiled plasmid DNA.

Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells Protocol Protocol reproducibly generates competent cultures of E. coli that yield 1 x 108 to 3 x 108 transformed colonies/µg of plasmid DNA. The protocol works optimally when the bacterial culture is grown at 18°C. If a suitable incubator is not available, a standard bacterial shaker can be set up in a 4°C cold room and regulated to 18°C.

Preparation of Electrocompetent E. coli Protocol Protocol for the preparation of electrocompetent E. coli.

Transformation of E. coli by Electroporation Protocol Protocol for the transformation of E. coli by electroporation. Includes: Preparation of E. coli cells for electroporation; Preparing DNA for Electroporation.