Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol Enzyme-linked reagents give excellent sensitivity and use a simple light microscope for detection. A range of enzymes is available, but for staining in situ, horseradish peroxidase will suit most needs. Diaminobenzidine (DAB) is one of the most sensitive substrates for horseradish peroxidase. It yields an intense brown product that is insoluble in both water and alcohol. It can be made more sensitive by adding metal salts such as cobalt or nickel to the substrate solution.
Mechanical Tracing of Protein Function in the Drosophila Ear Protocol Method relies on the examination of the ear’s mechanics, which is actively modulated by the motility of auditory neurons and reflects the function of mechanosensory proteins these cells comprise [5-7]. Mechanical signatures arising from the motility of the neurons are assayed by measuring the vibrations of the antennal sound receiver in the presence and absence of sound.