Amino-allyl Labeling This protocol describes the labeling of eukaryotic RNA with aminoallyl labeled
nucleotides via first strand cDNA synthesis followed by a coupling of the
aminoallyl groups to either Cyanine 3 or 5 (Cy 3/Cy5) fluorescent molecules. Hasseman. TIGR Microarr
Fabrication Protocol for DNA Microarrays This protocol describes the steps required to produce a cDNA microarray. Gene-specific DNA is produced by PCR amplification of purified template plasmid DNAs from cloned ESTs. The PCR product is purified by ethanol precipitation, thoroughly resuspended in
Labeled Probe Hybridization Procedure This protocol describes the hybridization of a Cy labeled cDNA probe (mix of Cy3 and Cy5) onto coated slide spotted with PCR amplified cDNA.
TIGR Microarray Protocols.
MAKING MICROARRAY PRINTING PLATES (IN DMSO) This protocol describes the method for making microarray printing plates in a 96
well format. (The same procedure applies to a 384 well format as well.) Hasseman. TIGR Microarray Protocols
MICROARRAY PCR, PURIFICATION, AND STORAGE This protocol describes PCR amplification of eukaryotic cDNA plasmid inserts,
gel electrophoresis, purification, and storage of PCR products. Hasseman. TIGR Microarray Protocols.
Microarray Protocols Heather R. Sanders.
Hybridization, Probe Creation and RNA preparation for Microarray Protocols.
Microarray Success The "7 keys to successful microarray analysis" has been designed to guide researchers step-by-step through the design, implementation, analysis, and publication of a microarray experiment.
Microarrays a Complete Manual PDF Microarray fabrication, coating, slide blocking, printing microarrays, RNA extraction, Sample Labeling for analysis, hybridization, complete protocol. NHGRI
This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.
DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.
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