DIG Biotin Labeled Probes Protocols

Chem-Link Labeling of DNA or RNA with DIG or Biotin Protocol Protocol for Chem-Link labeling of DNA or RNA with DIG or Biotin. Includes: Direct DIG Labeling of mRNA or cDNA with DIG Chem-Link; Estimating the Yield of DIG-labeled Nucleic Acids.

DIG Labeling of cDNA Probes Protocol Protocol for DIG labeling of cDNA probes. Includes: Incorporation DIG during PCR; Estimating the yield of DIG labeled probes; DNA Dot Blotting; Prehybridization and Hybridization.

DNA In Situ Hybridization with an Alkaline Phosphatase-Based Fluorescent Detection System Protocol Protocol describes a high sensitivity indirect detection procedure for DIG-labeled hybridization probes.  The procedure uses the components of the HNPP Fluorescent Detection Set to form a fluorescent precipitate of HNPP (2-hydroxy-3-naphthoic acid-2’-phenylanilide phosphate) and Fast Red TR at the site of hybridization.  This procedure can be used to detect single copy sequences as small as 1 kb on human metaphase chromosomes. 
   

Identification of Single Bacterial Cells Using DIG-Labeled Oligonucleotides Protocol Protocol for identification of single bacterial cells using DIG-labeled oligonucleotides. Includes: Organisms and growth conditions; Cell fixation and preparation of cell smears; DIG labeling of oligonucleotides with DIG-ddUTP; In situ hybridization using digoxigenin-labeled oligonucleotides.

In Situ Hybridisation to Alpha Satellite Sequences Protocol Protocol for in situ hybridisation to alpha satellite sequences (chromosome specific).

In Situ Hybridization to Human Metaphase Chromosomes Using DIG-, Biotin-, or Fluorochrome-Labeled Protocol for in situ hybridization to human metaphase chromosomes using DIG-, biotin-, or fluorochrome-labeled DNA probes and detection with fluorochrome conjugates.

Nick-Translation Labeling of ds DNA with DIG-, Biotin-, or Fluorochrome-Labeled dUTP Protocol Protocol for nick-translation labeling of ds DNA with DIG-, Biotin-, or Fluorochrome-labeled dUTP.

Nonradioactive In Situ Hybridization to Polytene Chromosomes With a DIG-Labeled DNA Probe Protocol Protocol for nonradioactive in situ hybridization to polytene chromosomes with a DIG-labeled DNA probe. Includes: Labeling the hybridization probe; Preparation and denaturation of polytene chromosomes from Drosophila, Chironomus, or other species; Hybridization and detection.

Oligonucleotide 3’-End Labeling with DIG-ddUTP or Biotin-ddUTP
Protocol   Protocol for oligonucleotide 3’-end labeling with DIG-ddUTP or Biotin-ddUTP.

Oligonucleotide Tailing With a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP Mixture Protocol Protocol for oligonucleotide tailing with a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP mixture.

PCR Labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes Protocol Protocol for PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes. Includes: PCR DIG labeling reaction for highly labeled probes containing unique sequences; PCR DIG labeling reaction for moderately labeled probes; PCR fluorescein labeling reaction for direct in situ probes.

Procedures for Labeling DNA, RNA, and Oligonucleotides with DIG, Biotin, or Fluorochromes Procedures for labeling DNA, RNA, and oligonucleotides with DIG, Biotin, or Fluorochromes. Includes: Random primed labeling of ds DNA with DIG-, Biotinor Fluorescein-High Prime reaction mix; PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit or PCR Labeling Mixes.

RNA Labeling by In Vitro Transcription of DNA with DIG, Biotin or Fluorescein RNA Labeling Mix Protocol for RNA labeling by in vitro transcription of DNA with DIG, biotin or fluorescein RNA labeling mix.