Cytotoxicity Protocols

Allium Test The Allium test provides a rapid screening procedure for chemicals, pollutants contaminants, etc. which may represent environmental hazards.  Root growth inhibition and adverse effects upon chromosomes provide an indication of likely toxicity.

CellTiter-Glo Luminescent Cell Viability Assay Protocol Protocol for CellTiter-Glo luminescent cell viability assay. This assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells.

Cytoskeletal Alterations as a Parameter for Assessment of Toxicity Changes in the balance of cytoskeletal proteins after exposure to test compounds can be detected by indirect immunofluorescence microscopy and quantitative biochemical methods.

CytoTox-ONE Homogeneous Membrane Integrity Assay Protocol Protocol describes automation of the CytoTox-ONE Homogeneous Membrane Integrity Assay.

Invitro Model for Prostoglandin H Synthase (PHS) Mediated Genotoxicity of Xenobiotics Protocol describes the use of SEMV cells (a cell line derived from ram seminal vesicles) in studies into prostaglandin H synthase-mediated metabolism of xenobiotics in intact cells.

Mammalian Toxicology Acute Toxicity Studies Mammalian toxicology for acute toxicity studies.  Includes: M101 Acute Limit Test (Rodents), M102 Acute Toxicity Study (Rats or Mice), M120 Dose Escalation Study (Dog), M103 Acute Dermal Limit Test, M104 Acute Dermal (LD50), Study M105 Primary Dermal Irritation, M106 Primary Eye Irritation Study, M106-A, M110 Local Lymph Node Assay in Mouse, M115 Rabbit Vaginal Irritation Assay.

Mammalian Toxicology Repeat-Dose Toxicity Studies Mammalian toxicology repeat dose toxicity studies. Includes: M204 14-Day Oral Toxicity Study (Rodents), M204-H, M204-RH, M205 14-Day Oral Toxicity Study (Dogs), M205-H, M205-RH, M201 28-Day Oral Toxicity Study, M201-R, M202 28-Day Oral Toxicity Study, M202-R, M211 90-Day Oral Toxicity Study (Rodent), M211-20, M212 90-Day Oral Toxicity Study (Dog), M215 90-Day Dermal Toxicity Study, M213 Two-Year Chronic Toxicity/Carcinogenicity Study, M214 6-Month Carcinogenicity Study in p53+/Transgenic Mice.

Molecular and Genetic Toxicology Studies for In Vitro Cytogenetics with CHO or human lymphocytes Molecular and genetic toxicology studies for in vitro cytogenetics with CHO or human lymphocytes. Includes: G401 Chromosomal Aberrations in Chinese Hamster Ovary (CHO) Cells, G401-2, G401-R, G401-R2, G401-R2H, G402 Chromosomal Aberrations in Human Lymphocytes, G402-2, G402-R, G402-R2, G402-R2H, G403 Sister Chromatid Exchanges in Chinese Hamster Ovary (CHO) Cells.

Molecular and Genetic Toxicology Studies for In Vivo Cytogenetics Molecular and genetic toxicology studies for in vivo cytogenetics. Includes: G501 Chromosomal Aberrations in Rodent Bone Marrow Cells, G502 Single-Exposure Bone Marrow Cytotoxicity Assay.

Molecular and Genetic Toxicology Studies on Gene Mutation in Mammalian Cells In Vitro Molecular and genetic toxicology studies on gene mutation in mammalian cells In vitro. Includes: G201 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Assay; G201-R; G202 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Screening Assay; G203 Chinese Hamster Ovary or V79 Cell Mutation Assay at the hprt Locus; G203R.

Molecular and Genetic Toxicology Studies on Microbial Mutagenesis Molecular and genetic toxicology studies on microbial mutagenesis. Includes: G101 Salmonella/Microsome Plate Incorporation Assay (Ames Test), G101-R, G102 Salmonella/Microsome Preincubation Assay (Ames Test), G102-R, G103 Salmonella/E.  Coli Reverse Mutation Assay (using four Salmonella and one E. coli strains), G104 Salmonella/E.  Coli Reverse Mutation Assay (using four Salmonella and two E. coli strains), G105, G106.

V79 Cytotoxicity Test for Membrane Damage The cytotoxic effect of test chemicals in V79 cell culture can be determined by assessing damage to the plasma membrane as determined by a nucleic acid leakage assay.