Protocols and information related to cytotoxicity. Includes: Cell Specific Cytotoxicity Assays; Cytotoxicity Assays in Cell Culture; Liver Toxicity Assays; Microorganism Cytotoxicity Assays.
Allium Test The Allium test provides a rapid screening procedure for chemicals, pollutants contaminants, etc. which may represent environmental hazards. Root growth inhibition and adverse effects upon chromosomes provide an indication of likely toxicity.
CellTiter-Glo Luminescent Cell Viability Assay Protocol Protocol for CellTiter-Glo luminescent cell viability assay. This assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells.
Molecular and Genetic Toxicology Studies for In Vitro Cytogenetics with CHO or human lymphocytes Molecular and genetic toxicology studies for in vitro cytogenetics with CHO or human lymphocytes. Includes: G401 Chromosomal Aberrations in Chinese Hamster Ovary (CHO) Cells, G401-2, G401-R, G401-R2, G401-R2H, G402 Chromosomal Aberrations in Human Lymphocytes, G402-2, G402-R, G402-R2, G402-R2H, G403 Sister Chromatid Exchanges in Chinese Hamster Ovary (CHO) Cells.
Molecular and Genetic Toxicology Studies on Gene Mutation in Mammalian Cells In Vitro Molecular and genetic toxicology studies on gene mutation in mammalian cells In vitro. Includes: G201 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Assay; G201-R; G202 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Screening Assay; G203 Chinese Hamster Ovary or V79 Cell Mutation Assay at the hprt Locus; G203R.
Molecular and Genetic Toxicology Studies on Microbial Mutagenesis Molecular and genetic toxicology studies on microbial mutagenesis. Includes: G101 Salmonella/Microsome Plate Incorporation Assay (Ames Test), G101-R, G102 Salmonella/Microsome Preincubation Assay (Ames Test), G102-R, G103 Salmonella/E. Coli Reverse Mutation Assay (using four Salmonella and one E. coli strains), G104 Salmonella/E. Coli Reverse Mutation Assay (using four Salmonella and two E. coli strains), G105, G106.
V79 Cytotoxicity Test for Membrane Damage The cytotoxic effect of test chemicals in V79 cell culture can be determined by assessing damage to the plasma membrane as determined by a nucleic acid leakage assay.