Competent Cells Protocols

Electro Competent Cells Protocol Protocol for electro competent cells. Preparation of Electro competent E. coli cells (per the BioRad directions).

Frozen Competent E. coli Preparation and Transformation Protocol This is a very easy protocol which yields transformation efficiencies appropriate for routine cloning and transformation.

High Efficiency FCC Preparation and Tx Protocol This is an easy and straightforward protocol that gives efficiencies of 106 to 107 cfu/mg of plasmid DNA.  A growth curve is required for each strain that is prepared.

Preparing Electrocompetent Cells Protocol Protocol for preparing electrocompetent cells.