Cell Cycle Protocols

Cell Cycle Analysis - Propidium Iodide Information on how to quantitatively assess DNA content. Includes: Propidium iodide staining protocol and staining of DNA by Mithramicin Protocol

Cell Cycle Protocol using Flow Cytometry Cell Cycle Protocol using Flow Cytometry. Cell cycle Analysis by Flow Cytometry. DNA content Analysis on the FACSAria. Analyzing Cell cycle FCM data. Biomedical Sciences Flow Cytometry Core Laboratory. Dr.Smith Cornell.

Cell Synchronization Using Centrifugal Elutriation Protocol protocol describes a method for the synchronization of cell populations using centrifugal elutriation. The method relies on the fact that cell size increases linearly as cells proceed through the cell cycle. Cells of similar size (and cell cycle phase) are eluted stepwise from the cell chamber, with the smallest size (those in early G1) being eluted first. Using this procedure, it is possible to obtain relatively pure populations of cells at various points in G1, S, and G2/M.

H-Thymidine Uptake by Cultured Cells Protocol Protocol for H-Thymidine uptake by cultured cells.

Preparing Cells for PI/FACS (cell cycle) Analysis Protocol This method works well to assess cell cycle distribution of whole cell populations. This method can also be used to assess the cell cycle distribution of GFP transfected cells however, the EtOH step is generally not sufficient to keep GFP in the cell.

Timing of Cycles Protocol Protcol for the timing of cycles.

Yeast Cell Cycle by Flow Cytometry Protocol Protocol for yeast cell cycle by flow cytometry. Includes procedure for dyes: Propidium Iodide and Sytox Green.