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Cell Biology and Cell Culture

Cell culture techniques. Cell Biology Experimental Methods. Subculture, transfection, splitting cells, cell count, cell viability. Basic cell culture protocols and methods. 

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Submitted Cell Biology and Cell Culture Articles

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

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