Construction of cDNA Libraries Protocol Here, the DNA-RNA hybrids synthesized in Stage 1 are converted into full-length double-stranded cDNAs. The primers for synthesis of second-strand cDNA are created by RNase H, which introduces nicks into the RNA moiety of the cDNA-mRNA hybrids. E. coli DNA polymerase I extends the newly created 3'-hydroxyl termini, using the first-strand cDNA as a template.
Screening Expression Libraries Constructed in Plasmid Vectors Protocol A cDNA library constructed in a plasmid expression vector of the pUC, pUR, or pEX series is plated on agar medium and then replicated onto filters, which are transferred to plates containing IPTG. After 2-4 hours of induction, the colonies are lysed with chloroform and then screened with appropriate antibodies.
C and M Law Corporation's
Los Angeles personal injury attorney firm has been serving the city’s residents for over 45 years. People who think they do not need the services of an experienced personal injury attorney, invariably find out the hard way that they should have chosen that right lawyer in the very beginning. Regardless of the type of accident or injury, we have the experience to successfully represent you and your family. If you or someone you know has been injured through the negligence or recklessness of others, come see us. Voted in the top one percent of trial lawyers in the USA, we can help get you the compensation you and your loved ones deserve. The personal injury attorney Los Angeles firm of C and M Law Corporation has won an excess of 2 Billion Dollars in settlements!