Assay of Intracellular Free Calcium in RAW 264.7 Cells This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+]i, in cultured adherent RAW 264.7 cells, using a 96-well plate format. This objective is accomplished by using the Ca2+-sensitive fluorescent dye, fluo-3, which permeates cell membranes as an ester and is
hydrolyzed in the cell to its Ca2+-sensitive acidic form.
Assay of Intracellular Free Calcium in Suspended B Cells This protocol describes a method to assess concentrations of free cytoplasmic calcium (Ca2+) in mouse splenic B cells in the absence and presence of ligands for cell surface receptors. This objective is accomplished with the Ca2+-sensitive fluorescent dye, Fluo-3, which permeates cells as an ester and is hydrolyzed in the cell to its Ca2+ sensitive acidic form.
Native Aequorin Native Aequorin. NanoLight Technology. Aequorin has advantages over other Ca2+ indicators, for example, low leakage rate from cells, lack of intracellular
compartmentalization or sequestration and it does not disrupt cell functions or embryo development.
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