Calcium Measurement Protocols

Assay of Intracellular Free Calcium in RAW 264.7 Cells This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+]i, in cultured adherent RAW 264.7 cells, using a 96-well plate format. This objective is accomplished by using the Ca2+-sensitive fluorescent dye, fluo-3, which permeates cell membranes as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form.

Assay of Intracellular Free Calcium in RAW 264.7 Cells Loaded with Fura-2 (with FLEXstation) This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+], in cultured adherent RAW 264.7 cells, using a 96-well plate format. This objective is accomplished by using the Ca2+-sensitive fluorescent dye, fura-2, which permeates cell membranes as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form.

Assay of Intracellular Free Calcium in Suspended B Cells This protocol describes a method to assess concentrations of free cytoplasmic calcium (Ca2+) in mouse splenic B cells in the absence and presence of ligands for cell surface receptors. This objective is accomplished with the Ca2+-sensitive fluorescent dye, Fluo-3, which permeates cells as an ester and is hydrolyzed in the cell to its Ca2+ sensitive acidic form.

Assay of Intracellular Free Calcium in Suspended B Cells Dual Ligand PDF The following protocol is a method to measure concentrations of free cytoplasmic calcium (Ca2+) in mouse splenic B cells in the presence of two ligands. Signalling Gateway

Assay of Intracellular Free Calcium in Suspended B Cells PDF Protocol for a method which assesses concentrations of free cytoplasmic calcium (Ca2+) in mouse splenic B cells in the absence and presence of ligands for cell surface receptors. Signalling Gateway

Expression of Recombinant Aequorin as an Intracellular Calcium Reporter PDF Shaw et al. Fungal Genetics and Biology 2001. first report of a plant pathogenic fungus for which aequorin-based Ca21 measurement protocols have been developed.

Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+ Protocol by using the technique of Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+. Protocol includes: Preparation of Indo-1 and preparation of Ionomycin.

Live Single-Cell Fura-2 Measurements to Determine the Intracellular Free Calcium in RAW 264.7 Cells This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+], for cultured adherent RAW 264.7 cells in an 8-well coverglass. This objective is accomplished using the Ca2+-sensitive fluorescent dye, fura-2 acetoxymethyl (AM), which permeates cell membranes as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form.

Measurement of Intracellular Calcium PDF Phenomenal review of the methods and references to intracellular calcium measurements. TAKAHASHI et al Phys. Rev. 1999

Native Aequorin Native Aequorin. NanoLight Technology. Aequorin has advantages over other Ca2+ indicators, for example, low leakage rate from cells, lack of intracellular compartmentalization or sequestration and it does not disrupt cell functions or embryo development.