C. elegans Staining Protocols

Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters.

Antibody Staining of Dissected Gonads Protocol Protocol for antibody staining of dissected gonads.

DAPI Staining Protocol Protocol for DAPI staining.

Double Dye Filling (using DiO and di-4 ANEPPS) Protocol Protocol for double dye filling (using DiO and di-4 ANEPPS).

Dye Filling to Stain Amphid and Phasmid Neurons Protocol Protocol for dye filling to stain amphid and phasmid neurons.

Nematode Dye Filling Protocol Protocol for nematode dye filling. Includes: Thumb plate; Microfuge tube; 15 ml tube; Viewing: Big Scope and Dissecting Scope.

PAR-1 Staining Procedure Protocol Protocol for PAR-1 staining procedure. Includes fixation and staining.

Phalloidin Staining of C. elegans Worms Protocol Protocol for phalloidin staining of C. elegans worms.

RUVKUN Antibody Staining Protocol Protocol for RUVKUN Antibody Staining. Includes: Fixation; To make solution; REDUCING DISULFIDES TO -SH; OXIDIZE -SH GROUPS TO -SO3; TO CHECK THAT WORMS ARE PERMEABLE TO MACROMOLECULES; ANTIBODY INCUBATIONS; PERMANENT SPRINGTIME MOUNTING.

Staining C. elegans for ß-galactosidase Protocol Protocol for staining C. elegans for ß-galactosidase.