Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters.
RUVKUN Antibody Staining Protocol Protocol for RUVKUN Antibody Staining. Includes: Fixation; To make solution; REDUCING DISULFIDES TO -SH; OXIDIZE -SH GROUPS TO -SO3; TO CHECK THAT WORMS ARE PERMEABLE TO MACROMOLECULES; ANTIBODY INCUBATIONS; PERMANENT SPRINGTIME MOUNTING.
C and M Law Corporation's
Los Angeles personal injury attorney firm has been serving the city’s residents for over 45 years. People who think they do not need the services of an experienced personal injury attorney, invariably find out the hard way that they should have chosen that right lawyer in the very beginning. Regardless of the type of accident or injury, we have the experience to successfully represent you and your family. If you or someone you know has been injured through the negligence or recklessness of others, come see us. Voted in the top one percent of trial lawyers in the USA, we can help get you the compensation you and your loved ones deserve. The personal injury attorney Los Angeles firm of C and M Law Corporation has won an excess of 2 Billion Dollars in settlements!