Fixation and Permeabilization of C. elegans Protocol Protocol for fixation and permeabilization of C. elegans. Protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite treatment and fixation are by themselves sufficient to open eggs.
Fixation and Staining Parameters Parameters for fixation and staining. Includes: Antigen, Fixation, Fixation time, Antibody and Dilution.
Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol Bouin’s fixative is a particularly good choice for worms because it penetrates dense tissues well and is extremely good for fixing antigens. Like all strong fixatives, however, it is unsuitable for some antibody-antigen pairs. In such cases, the length of time in the Bouin’s fixative can be shortened, or paraformaldehyde fixation can be used instead.
Fixing Caenorhabditis elegans in Paraformaldehyde Protocol Common method for fixing worms is to use paraformaldehyde. This method provides a gentler fixation than the Bouin’s method, but often requires the use of collagenase. This method is particularly good for examining adult worms.
