Buffers and Solutions

Written by Super User. Posted in Buffers

How to make commonly used solutions and buffers.

Commonly used chemicals, solutions, and buffers for the research laboratory. Alphabetically Arranged.

Buffers Recipes: Common buffer recipes to create for research and chemistry. Buffers include: PBS, Tris, LB, TBST, solutions and media.

EDTA Stock Solution 0.5 M

0.5 M EDTA Stock Recipe (100 mL) :

  • Dissolve 16.81 g of EDTA
  • Add distilled water to 90 mL
  • Adjust pH to 7.0
  • Adjust volume to 100 mL with Water

0.5M EDTA Storage : EDTA is stable at room temperature

PBS (Phosphate Buffered Saline)

PBS Stock Recipe (1 L) :

  • Dissolve in 800 mL of Water H20:
  • 80 g of NaCl 
  • 2 g of KCl
  • 26.8 g of Na2HPO4 - 7 H20
  •  2.4 g of KH2PO4
  • Adjust pH to 7.4 with 1M HCl
  • Adjust volume to 1 L with Water

PBS Storage : PBS is stable at room temperature, or can be stored at 4 degrees C.

Uses: PBS is a buffer commonly used to wash cells or western blot membranes.

TBS  (Tris Buffered Saline)

1X TBS Recipe (1 L) :

  • Dissolve 6.05g Tris and 8.76 NaCl in 800 mL of Water.
  • Adjust pH to 7.5 with 1M HCl
  • Adjust volume to 1 L with Water.
  • TBS Storage : TBS is stable at 4 degrees C for 4 months

    TBST  (Tris Buffered Saline Tween)

    1X TBST Recipe (1 L) : 1 mL of Tween-20 in 1 L of TBS buffer

    TBST Storage : TBST is stable at 4 degrees C for 4 months

    Uses: TBST is a buffer for washing western blot membranes.

    LB Broth   ( Luria Bertani )

    LB Broth Recipe (1 L) :

    • Dissolve 10 g Bacto-tryptone
    • 5 g Bacto-yeast Extract
    • 5 grams NaCl
    • Adjust volume to 1 L with Water
    • Put in Stir Bar and dissolve
    • Autoclave

    LB Broth : LB Broth is stable at room temperature R/T for several months. If there any signs of contamination or it becomes cloudy, discard and make fresh. display_block('buffers'); ?>