Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Bacteria Transformation Protocols

Protocols and information related to preparation and transformation of bacterial cells. 

Protocols » Bacteria Transformation Protocols

Bacteria Transformation Protocols Protocol Links

Submitted Bacteria Transformation Protocols Articles

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

[Read more]


Science News

For science news click here:Science News