Assembling Aggregates between Diploid and Tetraploid Embryos Protocol Protocol describes a method for producing diploid embryo-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for diploid embryo-diploid embryo aggregation. The resulting chimeras are useful for phenotypic analysis when an induced mutation has an extraembryonic phenotype.
Assembling Aggregates between Diploid Embryos Protocol Protocol describes a method for assembling aggregates between diploid embryos. If embryos from a heterozygous mutant intercross are aggregated with wild-type embryos, the resulting chimeras can be used for analyzing mutant phenotypes.
Assembling Aggregates between Embryonic Stem (ES) Cells and Diploid Embryos Protocol Protocol describes a method for assembling aggregates between ES cells and diploid embryos. The resulting chimeras are useful for separating certain extraembryonic phenotypes from phenotypes in the embryo proper, since the diploid embryo contributes to all parts of the conceptus, but the ES cell component does not contribute to the trophoblast or yolk sac endoderm.
Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos Protocol Protocol describes a method for producing ES cell-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for ES cell-diploid embryo aggregation in which diploid embryos are replaced with tetraploid embryos. The resulting chimeras can be used to analyze the embryonic versus extraembryonic phenotype of a mutation.
Preparing the Aggregation Plate Protocol Protocol describes the preparation of aggregation plates, which will then be used for the production of aggregation chimeras.
Removal of Zona Pellucida Protocol Protocol describes a simple method for removal of the zona pellucida of preimplantation mouse embryos using an acidified Tyrode’s solution.
