Arabidopsis Tissue Culture

Analysis of the Plant Epidermis Experiments Experiments included for analysis of plant epidermis: Steroid induction of gene expression in Arabidopsis; Replica Molds and Casts of the plant epidermis.

Arabidopsis Tissue Culture Stock Solutions and Media Arabidopsis Tissue Culture Stock Solutions and Media. Lazo Lab. MS MEDIUM FOR ARABIDOPSIS. B5 MEDIUM FOR ARABIDOPSIS. 10X Micronutrients.

Kanamycin Selection of Transformed Arabidopsis Protocol The most commonly used markers for selection of transgenic Arabidopsis are resistance to the antibiotic kanamycin and to the herbicide glufosinate ammonium. Resistance to kanamycin is conferred by a bacterial gene encoding the enzyme neomycin phosphotransferase (NPT). In this protocol, kanamycin-resistant seedlings are selected on solid medium.

Metabolic Labeling of Plant Cell Cultures with K15NO3 Protocol Protocol for metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites.

Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana. CL ENCINA1, M CONSTANTIN2 and J BOTELLA2. Plant Molecular Biology Reporter 19: 245–248, 2001.

Preparation of Arabidopsis Chloroplasts Protocol It is desirable to prepare subcellular fractions, either to localize proteins or to improve the sensitivity of protein detection. This procedure describes the enrichment of chloroplasts from Arabidopsis.

Preparation of Arabidopsis Mitochondria Protocol It is often desirable to prepare subcellular fractions, either to localize proteins or to improve the sensitivity of protein detection. This procedure describes the enrichment of mitochondria from Arabidopsis.

Procedure for Purifying Cell Walls from Arabidopsis Hypocotyls Protocol for the evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls.