Antibody Staining Protocols

Alkaline Phosphatase (APAAP) Technique Protocol Protocol for alkaline phosphatase (APAAP) technique. Includes: Fixation; Technique; Substrate Solution.

CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry Protocol for CD90 (Thy-1) antibody staining for Immunohistochemistry. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain.

High-Throughput Immunostaining Protocol Protocol for high-throughput immunostaining. Includes: [edit] Detection of 14-3-3 in U2OS and HeLa cells by immunofluorescence.

Histochemical Staining Protocol Protocol for histochemical staining. This protocol has been optimized for b-galactosidase and human placental alkaline phosphatase staining in retinal tissue and cultured cells.

Immunocytochemistry in Free-Floating Sections Protocol There are several strategies to visualize the antibody. For transmitted light microscopy, color development substrates for enzymes are often used. The antibody can be directly labeled with the enzyme. However, such a covalent link between an antibody and an enzyme might result in a loss of both enzyme and antibody activity. For these reasons several multistep staining procedures have been developed, where intermediate link antibodies are used. In this protocol use the Vectastain ABC-kit.

In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol Protocol for in situ hybridization for Epstein Barr virus-encoded RNA (EBER). Includes: Glassware decontamination; Double staining for EBER and a second antibody .

Preparing Cell Smears from Tissue Samples for Immunostaining Protocol Staining of tissues can be carried out on cell smears prepared from biopsy samples, such as needle aspirates, tissue scrapings, or freshly dissected tissues.

Preparing Frozen Tissue Sections for Immunostaining Protocol Frozen tissue sections show good preservation of tissue structure and antigens. The principle disadvantages of using them in immunostaining are that the specimens must be stored frozen, and a special microtome, known as a cryostat, is required. Also, many clinical specimens are not available in this form, and most classic histological descriptions of tissue structure and pathology are based on the use of paraffin-embedded sections of formalin-fixed material.

Preparing Paraffin Tissue Sections for Immunostaining Protocol Most histological studies are carried out on paraformaldehyde-fixed, paraffin-embedded tissue samples. Therefore, there is an extensive atlas of most tissues and organs prepared from these sources, and comparing the location of antigens to these data is immediately informative. The fixation and embedding procedures are harsh, however, and many antigens are not well preserved.

RUVKUN Antibody Staining Protocol Protocol for RUVKUN antibody staining. Includes: Fixation; To make solution; REDUCING DISULFIDES TO -SH; OXIDIZE -SH GROUPS TO -SO3; TO CHECK THAT WORMS ARE PERMEABLE TO MACROMOLECULES; ANTIBODY INCUBATIONS; PERMANENT SPRINGTIME MOUNTING.

VEGF Antibody Staining Protocol Protocol for VEGF antibody staining. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain.