Amino-allyl Labeling (GeneTAC) Protocol Protocol for amino-allyl labeling (GeneTAC). Includes: RT Reaction; Nucleotide Mix for one reaction; Coupling; Prehybridization of probe.
Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Protocol details the preparation of fluorescently labeled target samples (aminoallyl method) and hybridization of these samples to a microarray of Agilent inkjet-deposited presynthesized oligonucleotides. The procedure requires a minimum of 3 µg of purified total RNA as starting material.