#1  
Old 07-23-2013, 07:32 PM
Pipette Filler
Points: 54, Level: 1 Points: 54, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2013
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Cesium Chloride Gradient



Hello, all:

Anybody here have experience with running a cesium chloride gradient? I am trying to identify a particular protein and DNA from a cell culture.

Here's what I did: I took some media and cellular extract, added CsCl to a final concentration of 0.4 g/ml, and then spun this down at 50,000 rpm for 16 h. I took fractions from the gradient and measured the refractive index of the fractions with an Olympus refractometer.

My question is this - I was expecting some of the fractions to have a refractive index of somewhere between 1.30 and 1.35. Instead, all of the fractions were between 1.37 and 1.355. Did I do something wrong, was the CsCl gradient not separated enough, or was the RI numbers shifted because I used media from cells and extract rather than a more "pure" solution?

Please let me know. Thank you.
Reply With Quote
Reply


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Register Search Today's Posts Mark Forums Read

vBulletin Message
Hey there!

It appears that you've exceeded the maximum number of posts you can view, but wait, there's a simple solution. To unlock the forum and continue viewing messages, all you need to do is Sign Up for a FREE account. The entire process takes just a few Seconds! so Create your account and Start Learning Now!! and view as many threads as you like!

Already a member? LOGIN at the top of this page to stop seeing this message.

Forum Jump


All times are GMT. The time now is 02:27 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2012, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12203 seconds with 10 queries